5E3G
Crystal structure of the human BRPF1 bromodomain in complex with SEED8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99987 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.859, 60.859, 62.996 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.400 - 1.650 |
R-factor | 0.1977 |
Rwork | 0.197 |
R-free | 0.20520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lc2 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.765 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.400 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.040 | 0.442 |
Number of reflections | 16662 | |
<I/σ(I)> | 25.6 | 4.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.7 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Sodium Acetate, pH 5.5, 0.15 M Sodium Nitrate, 20% PEG3350 |