5E32
Crystal structure of H5 hemagglutinin mutant (N224K, Q226L, N158D and L133a deletion) from the influenza virus A/chicken/Vietnam/NCVD-093/2008 (H5N1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03314 |
Spacegroup name | P 6 |
Unit cell lengths | 133.046, 133.046, 134.693 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.327 - 2.700 |
R-factor | 0.1985 |
Rwork | 0.197 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gbm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.177 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.410 | |
Number of reflections | 33778 | |
<I/σ(I)> | 29.9 | |
Completeness [%] | 90.5 | 90.4 |
Redundancy | 6.2 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.2 M ammonium acetate, 20% (w/v) PEG3350 |