5E2E
Crystal Structure of Beta-lactamase Precursor BlaA from Yersinia enterocolitica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.116, 93.513, 122.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.720 - 1.900 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.906 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10PRE-2104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.720 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.753 | |
| Number of reflections | 38874 | |
| <I/σ(I)> | 16.8 | 1.75 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 4.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 289 | 0.1 M CHES:NaOH pH 9.5, 20% (w/v) PEG 8000 |
