5E1N
Selenomethionine Ca2+-Calmodulin from Paramecium tetraurelia qFit disorder model
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-04 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.036, 29.409, 52.793 |
| Unit cell angles | 89.45, 86.29, 82.39 |
Refinement procedure
| Resolution | 24.760 - 1.000 |
| R-factor | 0.135 |
| Rwork | 0.134 |
| R-free | 0.15000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1exr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.489 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.121 | 2.190 |
| Number of reflections | 75551 | |
| <I/σ(I)> | 11.5 | 1.6 |
| Completeness [%] | 94.0 | 84.7 |
| Redundancy | 5.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 50 mM sodium cacodylate, 50% MPD |
