5E1K
Selenomethionine Ca2+-Calmodulin from Paramecium tetraurelia SAD data
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-07-04 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 |
Unit cell lengths | 25.036, 29.410, 52.794 |
Unit cell angles | 89.45, 86.29, 82.39 |
Refinement procedure
Resolution | 25.000 - 1.000 |
R-factor | 0.144 |
Rwork | 0.143 |
R-free | 0.16500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1exr |
RMSD bond length | 0.013 |
RMSD bond angle | 0.029 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | SHELXL (2014) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.020 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.121 | 2.190 |
Number of reflections | 75551 | |
<I/σ(I)> | 11.5 | 1.6 |
Completeness [%] | 94.0 | 84.7 |
Redundancy | 5.5 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 50 mM sodium cacodylate, 50% MPD |