5E1J
Structure of voltage-gated two-pore channel TPC1 from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.430, 158.850, 217.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.712 - 3.308 |
R-factor | 0.3251 |
Rwork | 0.325 |
R-free | 0.33210 |
Structure solution method | SIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 0.854 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.360 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.800 | |
Number of reflections | 34119 | |
<I/σ(I)> | 36.1 | 1.6 |
Completeness [%] | 96.2 | 78.2 |
Redundancy | 6.5 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 26% PEG400, 150 mM BaCl2, 100mM HEPES |