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5E1F

Crystal structure of Staphylococcal nuclease variant Delta+PHS G20E at cryogenic temperature

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSEALED TUBE
Source detailsOTHER
Temperature [K]110
Detector technologyCCD
Collection date2015-08-06
DetectorAGILENT ATLAS CCD
Wavelength(s)1.5418
Spacegroup nameP 1 21 1
Unit cell lengths31.178, 60.353, 38.425
Unit cell angles90.00, 93.52, 90.00
Refinement procedure
Resolution38.350 - 1.900
R-factor0.1798
Rwork0.177
R-free0.22800
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3bdc
RMSD bond length0.019
RMSD bond angle1.819
Data reduction softwareCrysalisPro (1.171.36.32)
Data scaling softwareCrysalisPro (1.171.36.32)
Phasing softwarePHASER (2.5.6)
Refinement softwareREFMAC (5.8.0103)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.970
High resolution limit [Å]1.9001.900
Rmerge0.0250.130
Number of reflections11327
<I/σ(I)>39.626.4
Completeness [%]99.9100
Redundancy10.16.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP927735% MPD, 25 mM potassium phosphate, calcium chloride, pdTp

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