5E0Q

Crystal structure of the Nup98 C-terminal domain bound to nanobody TP377

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9787
Spacegroup name	P 41
Unit cell lengths	66.590, 66.590, 87.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.000 - 1.900
R-factor	0.1689
Rwork	0.167
R-free	0.19560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4krn
RMSD bond length	0.010
RMSD bond angle	1.068
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.010		1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.128	0.021	1.633
Rmeas	0.130	0.022	1.663
Total number of observations	823111
Number of reflections	30053	350	2177
<I/σ(I)>	27.72	158.69	2.59
Completeness [%]	99.5	99.4	98.7
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	45 % (w/v) Pentaerythritol propoxylate (17/8 PO/OH), 100 mM Tris