5E0C
Structural Insight of a Trimodular Halophilic Cellulase with a Family 46 Carbohydrate-Binding Module
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-11-19 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 120.143, 120.143, 205.327 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.451 - 2.351 |
R-factor | 0.1812 |
Rwork | 0.179 |
R-free | 0.23150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e09 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.985 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.451 | 2.435 |
High resolution limit [Å] | 2.351 | 2.351 |
Rmerge | 0.100 | 0.667 |
Number of reflections | 30948 | |
<I/σ(I)> | 19.4 | 2.5 |
Completeness [%] | 98.2 | 95.5 |
Redundancy | 7.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | 2.0 M ammonium sulfate, 5% (v/v) 2-propanol |