5E08

Specific Recognition of a Single-stranded RNA Sequence by an Engineered Synthetic Antibody Fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-17
Detector	PSI PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	199.515, 63.401, 57.061
Unit cell angles	90.00, 97.51, 90.00

Refinement procedure

Resolution	60.400 - 2.380
R-factor	0.2112
Rwork	0.209
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ivk
RMSD bond length	0.006
RMSD bond angle	1.427
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_1405)

Data quality characteristics

	Overall
Low resolution limit [Å]	60.400
High resolution limit [Å]	2.380
Rmerge	0.084
Number of reflections	28135
<I/σ(I)>	11.5
Completeness [%]	98.5
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	1.8 M Ammonium citrate