5E05
Structure of Sin Nombre virus nucleoprotein in shot-axis crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9798 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.742, 74.908, 154.359 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.438 - 2.300 |
R-factor | 0.1849 |
Rwork | 0.183 |
R-free | 0.22890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e06 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.097 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 13234 |
<I/σ(I)> | 31 |
Completeness [%] | 100.0 |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 200 mM HEPES (pH 7.5), 200 mM lithium sulfate monohydrate, 23% (w/v) PEG3350 |