5E03
Crystal structure of mouse CTLA-4 nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 42.147, 42.147, 208.517 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.561 - 1.685 |
| R-factor | 0.1716 |
| Rwork | 0.171 |
| R-free | 0.18930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ohq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.073 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.561 | 1.774 |
| High resolution limit [Å] | 1.685 | 1.685 |
| Rmerge | 0.056 | 0.218 |
| Number of reflections | 23330 | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 9.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | lithium sulfate, hepes |
