5E00
Structure of HLA-A2 P130
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-06 |
| Detector | SDMS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.608, 72.006, 123.788 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.826 - 1.700 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.20480 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.891 |
| Data reduction software | CNS |
| Data scaling software | CNS |
| Phasing software | CNS |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 381837 |
| <I/σ(I)> | 32.18 |
| Completeness [%] | 99.4 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1mol/liter bis-Tris (pH 6.5), 20% (wt/vol) polyethylene glycol 3350 |
