5DZZ
Structural characterization of intermediate filaments binding domain of desmoplakin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-11 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.931, 64.466, 74.044 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.620 - 2.600 |
R-factor | 0.2115 |
Rwork | 0.207 |
R-free | 0.26380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lm7 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.501 |
Data reduction software | SCALA |
Data scaling software | SCALA (3.0.15) |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1760) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 74.044 | 74.044 | 2.700 |
High resolution limit [Å] | 2.600 | 10.730 | 2.600 |
Rmerge | 0.090 | 0.046 | 0.316 |
Total number of observations | 54886 | 758 | 1020 |
Number of reflections | 18815 | ||
<I/σ(I)> | 10.6 | 26.6 | 2.4 |
Completeness [%] | 98.0 | 96.2 | 93 |
Redundancy | 2.9 | 2.8 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 22% PEG MME 5000, magnesium acetate, MES |