5DZX
Protocadherin beta 6 extracellular cadherin domains 1-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.040, 90.530, 258.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.973 - 2.879 |
| R-factor | 0.2439 |
| Rwork | 0.241 |
| R-free | 0.28860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zpl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.672 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.530 | 3.420 |
| High resolution limit [Å] | 2.879 | 2.879 |
| Rmerge | 0.110 | 0.280 |
| Number of reflections | 14851 | |
| <I/σ(I)> | 6.5 | 3 |
| Completeness [%] | 55.0 | 14.3 |
| Redundancy | 2.2 | 0.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | PEG 3350, Potassium thiocyanate |
