5DZX

Protocadherin beta 6 extracellular cadherin domains 1-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	49.040, 90.530, 258.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.973 - 2.879
R-factor	0.2439
Rwork	0.241
R-free	0.28860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zpl
RMSD bond length	0.003
RMSD bond angle	0.672
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.530	3.420
High resolution limit [Å]	2.879	2.879
Rmerge	0.110	0.280
Number of reflections	14851
<I/σ(I)>	6.5	3
Completeness [%]	55.0	14.3
Redundancy	2.2	0.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	PEG 3350, Potassium thiocyanate