5DZW
Protocadherin alpha 4 extracellular cadherin domains 1-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.480, 56.310, 173.670 |
| Unit cell angles | 90.00, 99.05, 90.00 |
Refinement procedure
| Resolution | 29.567 - 2.430 |
| R-factor | 0.2672 |
| Rwork | 0.266 |
| R-free | 0.29340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dzv |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.564 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.712 | 2.510 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.220 | 1.460 |
| Number of reflections | 14859 | |
| <I/σ(I)> | 4.8 | 0.9 |
| Completeness [%] | 92.0 | 82.1 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | Sodium chloride, Mes |
