5DZW
Protocadherin alpha 4 extracellular cadherin domains 1-4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.480, 56.310, 173.670 |
Unit cell angles | 90.00, 99.05, 90.00 |
Refinement procedure
Resolution | 29.567 - 2.430 |
R-factor | 0.2672 |
Rwork | 0.266 |
R-free | 0.29340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dzv |
RMSD bond length | 0.002 |
RMSD bond angle | 0.564 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.712 | 2.510 |
High resolution limit [Å] | 2.420 | 2.420 |
Rmerge | 0.220 | 1.460 |
Number of reflections | 14859 | |
<I/σ(I)> | 4.8 | 0.9 |
Completeness [%] | 92.0 | 82.1 |
Redundancy | 3.2 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | Sodium chloride, Mes |