5DZR

Structure of RNA Helicase FRH a Critical Component of the Neurospora Crassa Circadian Clock

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	76.797, 107.749, 125.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.826 - 3.161
R-factor	0.2664
Rwork	0.264
R-free	0.32520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xgt
RMSD bond length	0.003
RMSD bond angle	0.767
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	125.770	125.770	3.330
High resolution limit [Å]	3.160	10.000	3.160
Rmerge	0.143	0.026	1.438
Rpim	0.080	0.015	0.826
Total number of observations	71555	2426	9781
Number of reflections	18063
<I/σ(I)>	9.6	40.5	1.2
Completeness [%]	98.1	98.1	96.9
Redundancy	4	3.7	3.8
CC(1/2)	0.996	0.999	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	290	sodium citrate, PEG600