5DZN
human T-cell immunoglobulin and mucin domain protein 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 100.400, 107.297, 115.649 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.667 - 2.300 |
R-factor | 0.2019 |
Rwork | 0.200 |
R-free | 0.23780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.454 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.101 | 0.054 | 0.695 |
Rmeas | 0.110 | 0.059 | 0.758 |
Rpim | 0.043 | 0.024 | 0.300 |
Total number of observations | 373065 | ||
Number of reflections | 57162 | ||
<I/σ(I)> | 4.9 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 6.5 | 6.3 | 6.3 |
CC(1/2) | 0.995 | 0.794 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 20% (w/v) PEG 6000, 100 mM citric acid/sodium hydroxide, pH 5.0 |