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5DYV

AbyU - wildtype

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I24
Synchrotron siteDiamond
BeamlineI24
Temperature [K]193
Detector technologyPIXEL
Collection date2015-05-06
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.9730
Spacegroup nameP 1
Unit cell lengths61.800, 73.590, 86.190
Unit cell angles112.87, 90.04, 89.98
Refinement procedure
Resolution79.410 - 2.500
R-factor0.19946
Rwork0.197
R-free0.25298
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.015
RMSD bond angle1.848
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0107)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]79.410
High resolution limit [Å]2.3602.360
Rmerge0.1200.358
Number of reflections52922
<I/σ(I)>6.4
Completeness [%]92.4
Redundancy3.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.52910.02 M magnesium chloride, 0.1 M HEPES pH 7.5 18% w/v poly(acrylic acid) sodium salt MW 5100

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PDB entries from 2024-07-17

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