5DYR
Structure of virulence-associated protein D (VapD) from Xylella fastidiosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.4586 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 107.930, 107.930, 53.281 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.690 - 3.000 |
R-factor | 0.20183 |
Rwork | 0.199 |
R-free | 0.25306 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.722 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.100 |
High resolution limit [Å] | 2.200 |
Number of reflections | 15925 |
<I/σ(I)> | 6.7 |
Completeness [%] | 85.8 |
Redundancy | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 289 | 20% MPD, 0.1M NaAc pH 4.5, 170mM glycine |