5DYO
Fab43.1 complex with flourescein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.979710 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.232, 105.401, 109.059 |
| Unit cell angles | 90.00, 102.73, 90.00 |
Refinement procedure
| Resolution | 47.200 - 2.360 |
| R-factor | 0.1955 |
| Rwork | 0.193 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ocx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (BUSTER 2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.200 | 2.365 |
| High resolution limit [Å] | 2.360 | 2.357 |
| Rmerge | 0.104 | |
| Number of reflections | 49365 | |
| <I/σ(I)> | 10 | 2.1 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 297 | ammonium sulfate, sodium acetate |
