5DYF
The crystal structure of Aminopeptidase N in complex with N-benzyl-1,2-diaminoethylphosphonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | H 3 |
| Unit cell lengths | 224.644, 224.644, 57.938 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.650 - 1.854 |
| R-factor | 0.1474 |
| Rwork | 0.146 |
| R-free | 0.17440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gtq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.915 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_1888) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.650 |
| High resolution limit [Å] | 1.854 |
| Rmerge | 0.070 |
| Number of reflections | 119960 |
| <I/σ(I)> | 18.7 |
| Completeness [%] | 99.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 2.0 M Ammonium Sulfate |
