5DY0

Crystal of AmtR from Corynebacterium glutamicum in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 2 2 21
Unit cell lengths	101.731, 137.423, 239.874
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	137.420 - 3.000
R-factor	0.20677
Rwork	0.206
R-free	0.22398
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dy1
RMSD bond length	0.010
RMSD bond angle	1.385
Data reduction software	XDS
Data scaling software	Aimless (0.1.29)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	119.940	119.940	3.070
High resolution limit [Å]	3.000	14.070	3.000
Rmerge	0.055	0.028	0.683
Rpim	0.029	0.016	0.358
Total number of observations	295546	2155	20906
Number of reflections	67304
<I/σ(I)>	17.1	39.2	2.1
Completeness [%]	99.0	84.6	99.8
Redundancy	4.4	3.4	4.6
CC(1/2)	0.999	0.999	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	294	1.2 M Trisodium Citrate, 0.1 M NaHepes pH 6.8