5DXY
Crystal structure of Dbr2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.682, 80.430, 84.438 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.382 - 1.770 |
| R-factor | 0.1548 |
| Rwork | 0.153 |
| R-free | 0.18350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dxx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.038 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.4.0) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 4.800 | 1.770 |
| Rmerge | 0.066 | 0.027 | 0.298 |
| Total number of observations | 243177 | ||
| Number of reflections | 34967 | ||
| <I/σ(I)> | 15.7 | ||
| Completeness [%] | 99.8 | 99.2 | 99.8 |
| Redundancy | 7 | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 0.1M Bis-Tris, 25% PEG3350 |
