5DXW

Crystal structure of mouse PD-L1 nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 21 21 2
Unit cell lengths	85.191, 29.679, 35.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.230 - 1.441
R-factor	0.1695
Rwork	0.169
R-free	0.19340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ol0
RMSD bond length	0.006
RMSD bond angle	1.067
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	18.230
High resolution limit [Å]	1.441
Number of reflections	30989
<I/σ(I)>	23.4
Completeness [%]	99.3
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG 400, ammonium sulfate, hepes