5DXV
Crystal structure of Rethreaded DHFR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2015-06-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 44.817, 120.054, 111.187 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.550 |
R-factor | 0.1864 |
Rwork | 0.185 |
R-free | 0.21433 |
RMSD bond length | 0.024 |
RMSD bond angle | 2.284 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.550 |
Number of reflections | 40229 |
<I/σ(I)> | 24.9 |
Completeness [%] | 91.4 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.0M LiCl2, 0.1M Sodium acetate, and 30% PEG 6000 |