5DXI
Structure of C. albicans Trehalose-6-phosphate phosphatase C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97741 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.757, 87.200, 139.271 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.426 - 2.000 |
R-factor | 0.2011 |
Rwork | 0.200 |
R-free | 0.24160 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.099 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.053 | 0.020 | 0.527 |
Rmeas | 0.060 | 0.022 | 0.594 |
Rpim | 0.027 | 0.010 | 0.272 |
Total number of observations | 236274 | ||
Number of reflections | 49245 | ||
<I/σ(I)> | 11 | ||
Completeness [%] | 99.9 | 98.9 | 100 |
Redundancy | 4.8 | 4.6 | 4.7 |
CC(1/2) | 1.000 | 0.919 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris, 0.2 M lithium sulfate, 2 M ammonium sulfate |