5DX8

Crystal structure of CARM1, sinefungin, and methylated PABP1 peptide (R455)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 2
Unit cell lengths	74.862, 98.266, 207.545
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.940
R-factor	0.2059
Rwork	0.204
R-free	0.24590
RMSD bond length	0.010
RMSD bond angle	1.414
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.060
High resolution limit [Å]	1.940	1.940
Rmerge	0.120	0.771
Number of reflections	103564
<I/σ(I)>	11.5	2.6
Completeness [%]	90.0	92.5
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 18% w/v PEG 3350