5DWV
Crystal structure of the Luciferase complexed with substrate analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-02 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9791 |
Spacegroup name | P 41 |
Unit cell lengths | 73.101, 73.101, 96.465 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.430 - 2.300 |
R-factor | 0.1821 |
Rwork | 0.178 |
R-free | 0.22750 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.087 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10pre_2124: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.430 |
High resolution limit [Å] | 2.300 |
Number of reflections | 165729 |
<I/σ(I)> | 26.48 |
Completeness [%] | 100.0 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | Li2SO4, PEG8000, Tris |