5DWP
Crystal Structure of human transthyretin (TTR) processed with the CrystalDirect automated mounting and cryo-cooling technology
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 41.346, 62.521, 85.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.630 - 1.200 |
| R-factor | 0.16422 |
| Rwork | 0.163 |
| R-free | 0.19559 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pvl |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.732 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.630 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.068 | |
| Number of reflections | 69740 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 97.0 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium citrate, 1.6 M ammonium sulfate, pH=5.0 |
