5DVW
Structure of minor nucleoprotein V30 from Zaire ebolavirus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-09 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 |
Unit cell lengths | 34.090, 58.380, 66.600 |
Unit cell angles | 96.33, 90.11, 106.98 |
Refinement procedure
Resolution | 24.579 - 1.750 |
R-factor | 0.1686 |
Rwork | 0.167 |
R-free | 0.20780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i8b |
RMSD bond length | 0.010 |
RMSD bond angle | 1.095 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 7.830 | 1.800 |
High resolution limit [Å] | 1.750 | 5.530 | 1.750 |
Rmerge | 0.073 | 0.043 | 0.457 |
Rmeas | 0.095 | 0.056 | 0.598 |
Total number of observations | 114699 | ||
Number of reflections | 47118 | 943 | 3553 |
<I/σ(I)> | 8.9 | 19.05 | 1.96 |
Completeness [%] | 95.7 | 94.6 | 95.6 |
Redundancy | 2.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | Molecular Dimensions Morpheus screen e12: 40mM each Di-Ethyleneglycol, Tri-Ethyleneglycol, TetraEthyleneglycol, Penta-Ethyleneglycol; 12.% each MPD, PEG 1000; PEG 3350; 100mM Tris(base)/Bicine pH 8.5; EbzaA.17250.a.EW11.PD00370 at 22 mg/ml, tray 262514e12, puck otj5-3; cryo: direct |