5DVN

Fc K392D/K409D homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	193
Detector technology	CCD
Collection date	2014-07-25
Detector	RAYONIX MX-225
Wavelength(s)	0.97931
Spacegroup name	P 32 2 1
Unit cell lengths	107.603, 107.603, 56.973
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.1889
Rwork	0.186
R-free	0.24430
RMSD bond length	0.007
RMSD bond angle	1.175
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0017)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	93.187	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge		0.034	0.697
Rmeas	0.105
Rpim	0.033	0.012	0.342
Total number of observations	128970	4556	9799
Number of reflections	13146
<I/σ(I)>	18.8	51.3	2.3
Completeness [%]	97.9	99.6	86.3
Redundancy	9.8	9.7	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	100mM Tris pH 8.5 + 25% PEG MME 5K + 200mM Lithium Sulfate