5DVL

Fc Design 20.8.37 A chain homodimer Y349S/T366M/K370Y/K409V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	193
Detector technology	CCD
Collection date	2014-07-10
Detector	RAYONIX MX-225
Wavelength(s)	0.97931
Spacegroup name	P 32 2 1
Unit cell lengths	110.502, 110.502, 57.872
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.2017
Rwork	0.201
R-free	0.22240
RMSD bond length	0.008
RMSD bond angle	1.156
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0017)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.899	31.899	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge		0.064	0.742
Rmeas	0.145
Rpim	0.047	0.025	0.272
Total number of observations	297787	9290	42014
Number of reflections	32319
<I/σ(I)>	10.1	19.8	3.2
Completeness [%]	99.9	97.9	99.8
Redundancy	9.2	8.6	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	100mM Tris HCl pH 8.5 + 15% PEG 20K