5DVJ
Crystal structure of galactose complexed periplasmic glucose binding protein (ppGBP) from P. putida CSV86
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-11-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.650, 118.880, 66.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.810 - 1.800 |
| R-factor | 0.1798 |
| Rwork | 0.177 |
| R-free | 0.22936 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.902 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 76051 | |
| <I/σ(I)> | 19.97 | 3.66 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.1 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | 0.1 M phosphate citrate buffer, 2 M ammonium sulphate |
