5DV9

Crystal structure of the Luciferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-02
Detector	RAYONIX MX225HE
Wavelength(s)	0.9791
Spacegroup name	P 41 21 2
Unit cell lengths	117.967, 117.967, 95.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.708 - 2.400
R-factor	0.1946
Rwork	0.191
R-free	0.24130
RMSD bond length	0.008
RMSD bond angle	0.976
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2124: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.710
High resolution limit [Å]	2.400
Number of reflections	363262
<I/σ(I)>	32.13
Completeness [%]	100.0
Redundancy	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	Li2SO4, PEG 8000, Tris