5DUW
Crystal structure of the human galectin-4 N-terminal carbohydrate recognition domain in complex with lactose-3'-sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.180, 64.050, 64.650 |
| Unit cell angles | 90.00, 91.03, 90.00 |
Refinement procedure
| Resolution | 64.640 - 1.700 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i8t |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.3.6) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.640 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.072 | 0.369 |
| Number of reflections | 56576 | |
| <I/σ(I)> | 13.7 | 3.4 |
| Completeness [%] | 99.5 | 98.5 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 3.5 M sodium formate, 0.1 M Tris |
