5DUT

Influenza A virus H5 hemagglutinin globular head

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	93.590, 93.590, 48.376
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.600 - 1.500
R-factor	0.1695
Rwork	0.168
R-free	0.18950
RMSD bond length	0.007
RMSD bond angle	1.108
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.600
High resolution limit [Å]	1.500
Number of reflections	38181
<I/σ(I)>	32.2
Completeness [%]	98.2
Redundancy	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.08M Magnesium acetate tetrahydrate, 0.05M Sodium cacodylate trihydrate pH6.5, 30% w/v Polyethylene glycol 4000