5DUM
Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody 65C6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 63.539, 92.981, 134.005 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.130 - 3.003 |
R-factor | 0.218 |
Rwork | 0.215 |
R-free | 0.26870 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.383 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 16304 |
<I/σ(I)> | 13.9 |
Completeness [%] | 99.8 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 291 | 2% w/v Polyethylene glycol 400, 0.1M Imidazole pH7.0, 24% w/v Polyethylene glycol monomethyl ether 5000 |