5DUK

N-terminal structure of putative DNA binding transcription factor from Thermoplasmatales archaeon SCGC AB-539-N05

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97934
Spacegroup name	P 43 21 2
Unit cell lengths	43.179, 43.179, 194.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.465 - 2.352
R-factor	0.2258
Rwork	0.224
R-free	0.25340
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.494
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.10pre_2120)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.390
High resolution limit [Å]	2.350	6.370	2.350
Rmerge	0.125	0.081	0.780
Rmeas	0.128	0.084	0.814
Rpim	0.029	0.020	0.221
Total number of observations	164940
Number of reflections	8330
<I/σ(I)>	9.9
Completeness [%]	99.0	98.5	89.6
Redundancy	19.8	17.6	9.5
CC(1/2)		0.994	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	297	0.2 M ammonium Sulfate, 0.1M tri-Sodium chitrate, 15% PEG4000