5DUB
Context-independent anti-hypusine antibody FabHpu98 in complex with deoxyhypusine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-18 |
Detector | RAYONIX MX300HS |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 105.672, 293.976, 68.819 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.000 - 2.000 |
R-factor | 0.1829 |
Rwork | 0.181 |
R-free | 0.21960 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.344 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 74438 |
<I/σ(I)> | 16 |
Completeness [%] | 99.9 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 297 | 15% PEG8000 0.4M Li2SO4 |