5DU0
Crystal structure of rsFolder in the non-fluorescent off-state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.992 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.290, 134.800, 51.050 |
Unit cell angles | 90.00, 105.58, 90.00 |
Refinement procedure
Resolution | 95.770 - 2.350 |
R-factor | 0.21075 |
Rwork | 0.208 |
R-free | 0.25734 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.006 |
RMSD bond angle | 1.054 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.770 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 37525 | |
<I/σ(I)> | 10.1 | 2.2 |
Completeness [%] | 98.1 | 88.56 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris, 20% PEG3350 |