5DTY

Crystal structure of rsEGFP2 in the non-fluorescent off-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	51.195, 62.809, 70.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.910 - 1.500
R-factor	0.1807
Rwork	0.178
R-free	0.22380
RMSD bond length	0.018
RMSD bond angle	1.762
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.910	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	36769
<I/σ(I)>	11.9	1.2
Completeness [%]	98.1	91.59
Redundancy	4.6	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	293	0.1 M HEPES, 1.7 M ammonium sulphate