5DTU
Crystal structure of the RNA-helicase Prp28 from Chaetomium thermophilum bound to ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.910 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.500, 50.400, 65.800 |
| Unit cell angles | 90.00, 101.40, 90.00 |
Refinement procedure
| Resolution | 45.773 - 3.199 |
| R-factor | 0.2756 |
| Rwork | 0.274 |
| R-free | 0.28780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nho |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.992 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.773 | 3.299 |
| High resolution limit [Å] | 3.199 | 3.199 |
| Rmerge | 0.124 | 0.494 |
| Number of reflections | 8454 | |
| <I/σ(I)> | 9.17 | 2.45 |
| Completeness [%] | 95.1 | 85.1 |
| Redundancy | 3.58 | 3.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277.15 | 200 mM NaCl, 20 % (w/v) PEG 4000, 100 mM Tris pH 8.0 |
