5DTF
context-independent anti-hypusine antibody FabHpu98.61 in complex with hypusine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-20 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.95370 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.120, 75.770, 120.610 |
Unit cell angles | 90.00, 120.66, 90.00 |
Refinement procedure
Resolution | 37.216 - 1.900 |
R-factor | 0.2134 |
Rwork | 0.212 |
R-free | 0.25350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ds8 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.843 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.200 | 2.180 |
High resolution limit [Å] | 1.900 | 2.100 |
Rmerge | 0.362 | |
Number of reflections | 77821 | |
<I/σ(I)> | 9.9 | 2.2 |
Completeness [%] | 97.9 | 89.1 |
Redundancy | 3.4 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 297 | 24% PEG 1000 0.1M HEPES 7.6 10mg/ml |