5DTC
UBL Structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-07 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 35.298, 37.738, 41.134 |
Unit cell angles | 82.54, 64.31, 69.62 |
Refinement procedure
Resolution | 37.057 - 1.700 |
R-factor | 0.1778 |
Rwork | 0.175 |
R-free | 0.23180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wjs |
RMSD bond length | 0.006 |
RMSD bond angle | 0.930 |
Data reduction software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 18667 | |
<I/σ(I)> | 9.1 | 2.5 |
Completeness [%] | 95.3 | 85.4 |
Redundancy | 2.5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Sodium cacodylate, 1M Sodium Citrate |