5DT5
Crystal structure of the GH1 beta-glucosidase from Exiguobacterium antarcticum B7 in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.073, 104.599, 199.186 |
| Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
| Resolution | 49.110 - 2.240 |
| R-factor | 0.2103 |
| Rwork | 0.209 |
| R-free | 0.22870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ptv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.103 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.110 |
| High resolution limit [Å] | 2.240 |
| Rmerge | 0.103 |
| Number of reflections | 203452 |
| <I/σ(I)> | 9.54 |
| Completeness [%] | 97.4 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M TRIS (pH 8.5), 2% (v/v) PEG400 and 1.45 M lithium sulfate |
