5DSD

The crystal structure of the C-terminal domain of Ebola (Bundibugyo) nucleoprotein

Replaces:  5CIJ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-14
Detector	RAYONIX MX300HE
Wavelength(s)	0.99
Spacegroup name	P 64 2 2
Unit cell lengths	108.957, 108.957, 82.736
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	80.000 - 2.310
R-factor	0.1692
Rwork	0.168
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qb0
RMSD bond length	0.008
RMSD bond angle	1.253
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.084	0.432
Number of reflections	12398
<I/σ(I)>	20.3	1.9
Completeness [%]	94.3	64.7
Redundancy	14.8	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	Crystallization solution: 1.33 M LiSO4, 0.1 M Tris pH 8.5. 1:1 ratio (250 nL: 250 nL) of precipitant to protein (at a concentration of 13.9 mg/ml). 60 mcL reservoir. Protein purification buffer 50mM Tris-HCl, 150 mM NaCl; pH 7.5