5DSC

Context-independent anti-hypusine antibody FabHpu24.B in complex with hypusine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	69.082, 167.945, 84.927
Unit cell angles	90.00, 99.68, 90.00

Refinement procedure

Resolution	49.000 - 2.400
R-factor	0.2093
Rwork	0.207
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5drn
RMSD bond length	0.004
RMSD bond angle	0.913
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.071	0.415
Number of reflections	73996
<I/σ(I)>	15.4	2
Completeness [%]	100.0	95.2
Redundancy	1.9	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	14% PEG 6000 0.5M NaCl 10mg/ml