5DS8
Context-independent anti-hypusine antibody FabHpu98 in complex with hypusine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 106.005, 294.085, 68.737 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.200 - 1.950 |
R-factor | 0.1847 |
Rwork | 0.183 |
R-free | 0.22350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5drn |
RMSD bond length | 0.014 |
RMSD bond angle | 1.496 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.200 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.851 | |
Number of reflections | 78566 | |
<I/σ(I)> | 22 | 2.2 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 7.4 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG8000 0.5M Li2SO4 15.7mg/ml |