5DS7
2.0 A Structure of CPII, a nitrogen regulatory PII-like protein from Thiomonas intermedia K12, bound AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-10-13 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.870, 75.650, 80.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.566 - 2.000 |
| R-factor | 0.2318 |
| Rwork | 0.231 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.654 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.566 | 2.050 | |
| High resolution limit [Å] | 2.000 | 8.930 | 2.000 |
| Rmerge | 0.091 | 0.031 | 0.849 |
| Rmeas | 0.094 | 0.032 | 0.882 |
| Total number of observations | 304488 | ||
| Number of reflections | 21369 | 299 | 1474 |
| <I/σ(I)> | 22.79 | 67.02 | 3.47 |
| Completeness [%] | 96.7 | 98 | 92.8 |
| Redundancy | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 273 | 0.1 M Citric acid pH 4.0, 20% MPD |
